Lipoplex Thermodynamics: Determination of DNA-Cationic Lipoid Interaction Energies
نویسندگان
چکیده
منابع مشابه
Interaction of cationic porphyrins with DNA.
Utilizing linear dichroism (LD), circular dichroism (CD), and fluorescence energy transfer, the binding geometries of a series of Co(3+)-porphyrins and their free ligands were examined. The compounds studied were Co-meso-tetrakis(N-methylpyridinium-4-yl)porphyrin (CoTMPyP) and its free ligand (H2-TMPyP), Co-meso-tetrakis(N-n-butylpyridinium-4-yl)porphyrin (CoTBPyP) and its free ligand (H2TBPyP)...
متن کاملThermodynamics of twisted DNA with solvent interaction.
The imaginary time path integral formalism is applied to a nonlinear Hamiltonian for a short fragment of heterogeneous DNA with a stabilizing solvent interaction term. Torsional effects are modeled by a twist angle between neighboring base pairs stacked along the molecule backbone. The base pair displacements are described by an ensemble of temperature dependent paths thus incorporating those f...
متن کاملDNA interaction with freestanding cationic lipid bilayers
We study the interaction of ds-DNA molecules with freestanding and supported cationic lipid bilayers. DNA fragments of different lengths (5, 10, 20, and 48 kbp) were adsorbed on lipid membranes consiting mainly of zwitterionic DOPC with added fractions of cationic DOTAP (1...10 %). Freestanding bilayers were modeled using giant unilamellar vesicles of sizes >100 μm. Supported lipid bilayers wer...
متن کاملThermodynamics of cationic lipid binding to DNA and DNA condensation: roles of electrostatics and hydrophobicity.
Alkylammonium binding to DNA was studied by isothermal titration calorimetry. Experimental data, obtained as functions of alkyl chain length, salt concentration, DNA concentration, and temperature, provided a detailed thermodynamic description of lipid-DNA binding reactions leading to DNA condensation. Lipid binding, counterion displacement, and DNA condensation were highly cooperative processe...
متن کاملPhase behavior of cationic amphiphiles and their mixtures with helper lipid influences lipoplex shape, DNA translocation, and transfection efficiency.
Cationic lipids are widely used for gene transfection, but their mechanism of action is still poorly understood. To improve this knowledge, a structure-function study was carried out with two pyridinium-based lipid analogs with identical headgroups but differing in alkyl chain (un)saturation, i.e., SAINT-2 (diC18:1) and SAINT-5 (diC18:0). Although both amphiphiles display transfection activity ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2003
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(03)74811-5